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Dans notre pratique de médecine générale, nous effectuons chaque jour un ou plusieurs prélèvements bactériologiques ou mycologiques en vue d’obtenir des réponses à des interrogations cliniques concernant les affections de nos patients. La prescription et la réalisation de ces prélèvements paraissent souvent anodines. Cependant, les multiples petites erreurs que nous commettons au quotidien ont un retentissement important sur l’interprétation finale des résultats, qui devient parfois inexploitable et nécessite la répétition de l’analyse. À travers cet ouvrage, je souhaite sensibiliser le médecin généraliste sur les erreurs qu’il commet fréquemment, que ce soit en termes de prescription ou de technique de prélèvement, pour ensuite développer des pistes et des outils pratiques (fiches didactiques et checklists) lui permettant de s’améliorer. Mais je désire aussi lui faire part de mes apprentissages en vue de rendre sa pratique plus confortable, en l’invitant à se familiariser avec de nouvelles connaissances théoriques et matérielles, dont certaines ont été approuvées par des pairs.

Specimen Handling --- Bacteriological Techniques --- Checklist --- General Practice --- Prescriptions

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Diagnostic techniques and procedures --- Laboratory techniques and procedures --- Specimen handling --- Dogs --- Cats --- veterinary

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Laboratory techniques and procedures --- Specimen handling --- Ferrets --- Rabbits --- Rodentia --- Birds --- Reptiles --- veterinary --- veterinary

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The human immunodeficiency virus (HIV) is characterized by extremely high variability resulting in the emergence of widely divergent viral strains in diverse geographical locations and different populations. HIV strains can also vary significantly with regard to their biological and immunological properties, which may have important implications for clinical aspects of HIV infection, diagnostics, treatment, and the development of effective HIV vaccines. It is therefore important to develop appropriate laboratory technologies and capacities for systematic collection and detailed characterization of globally prevalent HIV-1 strains. This second edition contains the latest information and recommendations with regard to standard procedures for HIV isolation and its genetic, biological, and immunological characterization with a special emphasis on their applicability in HIV vaccine-related research. The laboratory methods described in these guidelines were intensively validated through various collaborative studies conducted in the framework of the WHO-UNAIDS Network for HIV Isolation and Characterization.

HIV --- HIV --- Laboratories --- AIDS Vaccines. --- Containment of Biohazards. --- Specimen Handling. --- genetics. --- isolation & purification. --- standards.

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Dyspepsia --- Helicobacter pylori --- Helicobacter Infections --- Gastritis --- Specimen Handling --- microbiology --- isolation & purification --- blood --- drug therapy --- methods